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Highly Efficient ChIP Assay Kit for FFPE Samples

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Chromatrap® has introduced a new Formaldehyde Fixed Paraffin-Embedded (FFPE) ChIP Kit which overcomes the widely acknowledged shortfalls of the technique.

Due to the cross-linking of proteins by formaldehyde, FFPE tissues have traditionally presented a particular challenge for ChIP analysis. In addition, the small size and delicate nature of the tissue, and the difficulty in extracting samples from paraffin, used to provide tissue support after preservation, can damage complex cellular complexes such as chromatin. ChIP assays from these samples until now have therefore proved time consuming and difficult.

Available in 96-well or spin column formats the new Chromatrap® FFPE ChIP kit not only provides greater flexibility and more IPs per sample but is also proven to work well across a range of human and animal FFPE samples.

Requiring 10x less starting material compared to traditional methods the Chromatrap® FFPE ChIP kit produces 4x better pull down than even the best competitive kits. The new Chromatrap® FFPE ChIP kit works equally well with high or low abundant marks and provides sufficient DNA to perform library preps for ChIP-seq assays.