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IDT Introduces Downloadable qPCR User Guide

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Integrated DNA Technologies (IDT) has developed an extensive quantitative real-time polymerase chain reaction (qPCR) user guide, which is available to download for free from www.idtdna.com.

Providing user guidance on the entire qPCR process - from RNA isolation to data analysis - the manual covers the basics of experimental set-up, performance and analysis. Specific information on 5’ nuclease assays, including re-suspensions and qPCR protocols are also supplied, as well as a troubleshooting section which discusses commonly encountered issues. The entire document is written in compliance with MIQE guidelines: minimum information for publication of quantitative real-time PCR.