We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
IDT Launches TriFECTa™ Kit
Product News

IDT Launches TriFECTa™ Kit

IDT Launches TriFECTa™ Kit
Product News

IDT Launches TriFECTa™ Kit


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "IDT Launches TriFECTa™ Kit "

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Integrated DNA Technologies (IDT), has announced the launch of the TriFECTa™ Dicer-Substrate RNAi Kit.

Employing 27-mer duplex RNA, TriFECTa delivers a significant design improvement over first-generation 21-mer based RNAi kits.

Processed by Dicer into 21-mer siRNAs, IDT's 27-mer RNAi design delivers up to a 100-fold increased potency when compared to older 21-mer siRNA models at the same site.
 
Utilizing the endogenous Dicer system, IDT's TriFECTa represents a substantial improvement over traditional antisense based processes, and gives researchers the confidence they need when studying genes.

Backed by the companies functional knockdown guarantee, at least one of the kit's three Dicer-Substrate RNAi duplexes will deliver a 70% knockdown of the target mRNA when 90% transfection efficiency, and 90% knockdown with positive control is demonstrated.

“Designing RNAi duplexes that engage and exploit Dicer's excellent natural capabilities rather than avoiding it using siRNAs enables researchers to significantly raise efficacy - often at sub-nanomolar concentrations,” noted Dr. Mark Behlke, vice president of molecular genetics at IDT.

“Our research with City of Hope revealed that researchers can achieve dramatically improved levels of RNAi-mediated gene knockdown at significantly lower concentrations than was previously possible.”

The TriFECTa kit includes three target-specific Dicer-Substrate siRNA duplexes (5 nmoles each), Cy3TM DS Transfection Control, fluorescent-labeled transfection control duplex (1 nmole), HPRT-S1 DS Positive Control duplex (1 nmole), DS Scrambled-Neg, universal negative control duplex (1 nmole), RNase Free Duplex Buffer (100mM KAc/30 mM HEPES pH 7.5).

Advertisement