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Illumina Introduces Revolutionary Workflow Enhancements for MiSeq™


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Illumina has announced three innovative workflow solutions to support its MiSeq personal sequencer system. Designed for an optimal user experience, these products deliver a sequencing ecosystem that is unique in its level of integration and ease of use.

The three additions to the MiSeq ecosystem are:

• TruSeq Custom Amplicon Kits, offering a scalable, multiplexed assay for rapid and cost-effective variant identification. Users can, in less than eight hours, prepare and enrich up to 384 amplicons per sample and up to 96 samples per plate simultaneously using standard lab equipment;

• New Nextera™ DNA Sample Preparation Kits, delivering sequencing's fastest and easiest sample prep solution for a range of DNA sequencing applications in 90 minutes, less than a quarter of the time required for other platforms; and

• BaseSpace™, a secure and customizable cloud computing environment that eliminates the need for onsite infrastructure by offering unlimited storage and computing power with data management, archive, analysis, and real-time sharing capabilities.

"The MiSeq platform continues to redefine the personal sequencer experience by providing researchers with complete, simple, and cost-effective solutions," said Jay Flatley, CEO of Illumina.

Flatley continued, "Whether you are new to sequencing or currently use capillary electrophoresis, the TruSeq Custom Amplicon Kit features a workflow that can seamlessly address your targeted sequencing needs. The addition of BaseSpace™ eliminates the need for expensive IT infrastructure, simplifying the process of adopting a personal sequencer for labs of any size and experience. We are excited about the ability of these products to remove significant barriers for technology adoption and accelerate the migration of sequencing from capillary electrophoresis to our next-gen platforms."

"The MiSeq was by far the best 'plug and play' instrument we've tested in over 20 years of instrumentation evaluation," said Elaine Mardis, Professor in Genetics and Molecular Microbiology Co-director, The Genome Institute at Washington University.

Mardis continued, "Within a week, we went from crate to installation and generated four runs with high data quality - paired 150 base pair reads with 80% of bases greater than Q30. The high-quality, one-day turnaround will facilitate our applications development and bring next-generation sequencing one step closer to the clinic."

With Nextera and MiSeq users can go from DNA to analyzed data in less than eight hours. For custom amplicon sequencing, TruSeq Custom Amplicon Kits provide a highly multiplexed and cost-effective workflow, allowing studies that are not feasible using capillary electrophoresis sequencing methods to be completed in as little as two days with no additional hardware required.

Researchers can design a custom assay to target up to 384 genomic regions in a single experiment using Illumina's DesignStudio™, an intuitive, online tool. In addition, pre-configured onboard data analysis allows for easy variant interpretation.

"We recently ran our samples on MiSeq using the TruSeq Custom Amplicon assay and generated excellent quality sequence data," says Sheila Fisher, Assistant Director of Technology Development at the Broad Institute.

Fisher continued, "The assay was really easy to use and generated deep and highly uniform coverage across our selected targets. We were particularly impressed with the assay's ability to deliver stunning specificity for the level of multiplexing in a single well. We think that Illumina's TruSeq Custom Amplicon Kits will be a highly cost-effective and scalable method for targeted re-sequencing projects looking at small to medium numbers of amplicons across 96 samples on a single MiSeq run."

In addition to onboard analysis on MiSeq, users now have the option to selectively share their results with scientists around the world using BaseSpace. BaseSpace is a secure, extensible cloud-based resource for managing all of a customer's data and analyses without the need for onsite computing infrastructure.

MiSeq data can be seamlessly transferred to BaseSpace for scalable data storage, analysis, and sharing between researchers and their peers around the world, in a secure and user-friendly environment.

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