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Illumina Launches Suite of Next-Generation Sequencing Kits
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Illumina has announced the release of new sequencing chemistry kits and complementary software for its Genome Analyzer system. These new kits and software enable researchers to generate 40% more reads per run and extend read length to greater than 75 base pairs (bp).
Also launched is the new Mate Pair Library Preparation Kit, which provides support for generating longer insert paired-end libraries and is complementary to Illumina’s existing short-end paired libraries. These new improvements enable researchers to generate 10 to 15 Gigabases (Gb) of high-quality data per run, more than doubling the output previously attainable on the Genome Analyzer.
“The availability of mate pair library kits and long paired-end reads has greatly increased the flexibility and capacity of our Illumina sequencers. I believe that they have greatly improved our ability to sequence cDNA libraries and may even open up the possibility to do de novo sequencing on the Illumina sequencer,” said W. Richard McCombie, Ph.D., Professor at the Cold Spring Harbor Laboratory. “They are also greatly helping our medical resequencing by giving us more data and the ability to look for small insertions and deletions in patient samples.”
“In addition to providing new solutions for de novo sequencing, the combination of short insert paired-end reads with the new longer insert mate pair sequencing is the most powerful approach for maximal coverage across the genome. This combination enables detection of the widest range of structural variant types and is essential for accurately identifying complex rearrangements,” said David Bentley, Vice-President and Chief Scientist of DNA Sequencing at Illumina.
Also launched is the new Mate Pair Library Preparation Kit, which provides support for generating longer insert paired-end libraries and is complementary to Illumina’s existing short-end paired libraries. These new improvements enable researchers to generate 10 to 15 Gigabases (Gb) of high-quality data per run, more than doubling the output previously attainable on the Genome Analyzer.
“The availability of mate pair library kits and long paired-end reads has greatly increased the flexibility and capacity of our Illumina sequencers. I believe that they have greatly improved our ability to sequence cDNA libraries and may even open up the possibility to do de novo sequencing on the Illumina sequencer,” said W. Richard McCombie, Ph.D., Professor at the Cold Spring Harbor Laboratory. “They are also greatly helping our medical resequencing by giving us more data and the ability to look for small insertions and deletions in patient samples.”
“In addition to providing new solutions for de novo sequencing, the combination of short insert paired-end reads with the new longer insert mate pair sequencing is the most powerful approach for maximal coverage across the genome. This combination enables detection of the widest range of structural variant types and is essential for accurately identifying complex rearrangements,” said David Bentley, Vice-President and Chief Scientist of DNA Sequencing at Illumina.
