Increased Sample Throughput on Applied Biosystems Genomic Analysis Platform
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Applied Biosystems has announced the commercial availability of a new small RNA expression analysis solution optimized for the SOLiD™ System, the company’s ultra-high throughput platform for DNA sequencing and genomic analysis research.
This solution includes a multiplexing technology (also known as barcoding) that enables the simultaneous analysis of up to 256 biological samples in a single sequencing run. The multiplexing technology is currently available in limited release through an early access programme.
According to Company, this new multiplexing technology is expected to simplify the workflows and reduce the experimental time and cost of applications such as studying the function of small RNA molecules, validation of whole genome association results, targeted resequencing, chromatin immunoprecipitation (ChIP), rare and somatic mutation detection, and epigenetic studies.
Use of this multiplexing technology is expected to reduce both labor and reagent costs for researchers preparing samples for the SOLiD System by allowing them to pool multiple libraries of nucleic acid fragments, which reduces the number of emulsion PCR reactions. These pooled samples can then be sequenced in a single reaction.
The combination of the SOLiD System’s high sample and sequence throughput allows researchers to use each pooled, labelled set of samples in different slide configurations, enabling the ability to sequence 256 samples in a single sequencing run.
In addition to the multiplexing technology, the SOLiD System’s 2-base encoding benefits microRNA profiling studies. In profiling of microRNAs, sequencing allows researchers to discover variants that may occur in different species of these molecules, which can greatly influence the function of the microRNA molecule.
