Invitrogen Launches New RNAi Vectors

Invitrogen Corporation announced the launch of two new vectors for RNAi research. The BLOCK-iT™ HiPerform™ Lentiviral PolII miR RNAi Expression System with Emerald Green Fluorescent Protein (EmGFP) offers the expected ability to deliver to nondividing, primary, and hard-to-transfect cells with lentiviral technology coupled to multi-site technology for promoter flexibility making it ideal for difficult applications including in vivo RNAi. The new vector contains an mRNA stabilizing sequence (WPRE) and a nuclear import sequence (cPPT) which generate up to 5-fold higher virus titers and emGFP expression than previous versions. The BLOCK-iT™ Inducible PolII miR RNAi Expression System uses a tetracycline-inducible promoter that allows researchers to control miR RNAi expression so that even genes that are essential to the function of a cell may be knocked down.