Invitrogen Offers TC-FlAsH Expression Analysis Detection Kits for Specific Pre-Gel Protein Staining

Invitrogen Corporation has launched its new TC-FlAsH™ Analysis Detection Kits for in-gel detection of protein expression without any gel staining or gel handling required post-electrophoresis.

The TC-FlAsH kits utilize the green fluorescent FlAsH-EDT2 reagent to directly label tetracysteine (TC) tagged proteins. The kits’ simple protocol permits the use of crude or purified cell lysate, and requires only thirteen minutes of preparation time to stain proteins prior to gel loading. After electrophoresis, TC-FlAsH labeled proteins are detectable in-gel, or even in-cassette, via fluorescent imaging with western blot-like specificity and sensitivity. Total protein is visualized in contrasting stain of either red or orange, alongside the Benchmark™ total protein ladder for reference. The TC tag is small in size for minimal disruption of the native protein structure and function, and the fluorogenic labeling is reversible.