Kits for Reproducible mRNA Amplification
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ExpressArt® mRNA Amplification Kits from AMSBIO offer an elegant technological solution for qRT-PCR and gene expression array studies that overcomes the limitations of sample quality and quantity.
ExpressArt® technology allows reproducible analysis of challenging samples including Formalin-Fixed Paraffin-Embedded (FFPE), laser captured micro-dissections, needle biopsies, and circulating tumor cells.
Based on a non-Eberwine method using patented TRinucleotide primers and reagents - ExpressArt® minimizes loss of sequence associated with two round amplification procedures and eliminates 3' bias in amplified products. The resultant RNA produces data on differential expression of low copy number genes that fail to give sufficient signal intensities using alternative methods, allowing direct comparison of data from a range of input sample sizes. In addition using this exclusive primer technology - ExpressArt® is able to amplify severely degraded RNA sequences enabling analysis of RNA with poor RIN values.
An optimized ExpressArt® kit is available that allows specific amplification of bacterial mRNA from any species without the need for additional procedures to deplete ribosome RNA. Together with amino-allyl labeling modules and exclusive reagents for enhancing RNA isolations from very small and FFPE samples, ExpressArt® provides solutions to problems no other amplification technology has overcome.
ExpressArt® technology allows reproducible analysis of challenging samples including Formalin-Fixed Paraffin-Embedded (FFPE), laser captured micro-dissections, needle biopsies, and circulating tumor cells.
Based on a non-Eberwine method using patented TRinucleotide primers and reagents - ExpressArt® minimizes loss of sequence associated with two round amplification procedures and eliminates 3' bias in amplified products. The resultant RNA produces data on differential expression of low copy number genes that fail to give sufficient signal intensities using alternative methods, allowing direct comparison of data from a range of input sample sizes. In addition using this exclusive primer technology - ExpressArt® is able to amplify severely degraded RNA sequences enabling analysis of RNA with poor RIN values.
An optimized ExpressArt® kit is available that allows specific amplification of bacterial mRNA from any species without the need for additional procedures to deplete ribosome RNA. Together with amino-allyl labeling modules and exclusive reagents for enhancing RNA isolations from very small and FFPE samples, ExpressArt® provides solutions to problems no other amplification technology has overcome.
