Kreatech Introduces Enabling Technology for Labeling FFPE Samples for ArrayCGH BAC Arrays
Product News Jun 07, 2007
Kreatech Biotechnology BV announced the release of an enabling method for labeling DNA isolated from FFPE samples ArrayCGH analysis of particular samples, such as FFPE tissue, can be challenging due to the quality of DNA samples available.
DNA isolated from FFPE material is often highly degraded and the quality is usually low due to the presence of cross-links in the DNA. This makes the labeling procedure difficult and susceptible to failure.
The ULS arrayCGH Labeling Kit delivers a procedure for the direct (non-enzymatic) labeling of DNA. This method has now been optimized to provide a reproducible labeling procedure of FFPE DNA samples for arrayCGH analysis on BAC arrays. This kit also includes the KREApure purification columns.
These columns specifically remove unreacted ULS reagent from the labeling reaction but have no affinity for nucleic acids. This makes the recovery extremely high and without size exclusion, making it ideal for work with fragmented (FFPE) DNA samples.
“The ultimate benefit of the ULS technology for FFPE samples is that it does not require enzymes to label the DNA”, said Dimitri Pappaioannou, Product Manager at Kreatech.
"Also, since the abeling reaction is independent of the fragment length of DNA, ULS labeling is very suited for labeling small fragments of DNA and it provides researchers with an easy and reproducible method for arrayCGH analysis of FFPE samples,” Pappaioannou added.
Kreatech’s ULS technology relies on the special binding properties of platinum, to form coordinative bonds with biomolecules by binding to specific sites on DNA, RNA and proteins. In this way, ULS acts as relatively simple molecular glue for DNA, RNA and proteins. As a result of its versatility, customers have a choice of the molecular label attached to the ULS they can use to label the biomolecules of interest.