Kreatech Launches Low Input miRNA Isolation and Labeling Kit
Product News Jun 04, 2007
Kreatech Biotechnology has announced the release of the latest in a line of labeling kits for miRNA analysis applications the “miRacULS II: Low Input miRNA Isolation and Labeling Kit”.
This kit combines the isolation of the miRNA from tissue or cultured cells and the reagents necessary to label these samples for microarray analysis. The miRacULS II Kit is available with the ULS labeling molecule bound to Cy3 and Cy5 dyes licensed from GE Healthcare.
The discovery of miRNAs that have regulatory effects on gene expression has excited researchers to elucidate and characterize the function of these molecules in molecular processes and as possible tools in drug discovery.
One common way to examine miRNAs, is to analyze them on a microarray. Due to their small size, these RNA molecules prove very difficult to label via the enzymatic reactions. The ULS labeling technology it is a non enzymatic labeling reaction and is completely independent of fragment size, Therefore, ULS enjoys a unique advantage because it can just as efficiently labels small RNA molecules as it does all nucleic acids.
The miRacULS II kit is an expansion Kreatech’s miRNA product line and provides a solution to isolate and label miRNAs from limited amount samples (less than 105 cells or 5 mg tissue) or samples with a low miRNA expression level. This kit also includes the unique KREApure purification columns. These columns specifically remove unreacted ULS reagent from the labeling reaction but have no affinity for nucleic acids. This makes the recovery high and without size exclusion.
“Our miRacULS II kit expands our product offering for microarray based miRNA analysis. By supplying this combined isolation and labeling kit we provide our customers with convenient, rapid and reproducible kits to carry out their miRNA experiments when starting from limited amounts of input material. In the emerging field of miRNA analysis by Microarray, ULS proves to be a powerful enabling tool where other enzymatic methods often fail” according to Dimitri Pappaioannou, Product Manager at Kreatech.