Low Volume Genomic Analysis
Product News Jul 28, 2009
Using Jenway’s Genova spectrophotometer together with a Hellma TrayCell fibre-optic cuvette enables sample volumes as low as 0.7µl to be analyzed with high reproducibility.
Optimized for use by life science research chemists, the Genova is a true scanning UV/Visible spectrophotometer incorporating five pre-programmed methods for DNA/RNA analysis.
Measurement of DNA concentrations and purity ratios can be obtained using the wavelengths recorded at 260 and 280nm or 260 and 230nm, with optional correction at a third wavelength. The purity scan gives a clear, graphic display of DNA purity.
The TrayCell cuvette with integrated beam deflection and fibre-optic cables has two caps creating light paths of 1mm or 0.2mm – equivalent to a ‘virtual dilution’ of 1:10 or 1:50 when compared to measurements with a standard 10mm cuvette.
The volume range for TrayCell is as low as 0.7–5µl, compared with a lowest sample volume of 10ml using a standard cuvette. After measurement, samples are simply wiped from the TrayCell’s optical window while the cuvette remains in place in the instrument, thus saving time and ensuring that the aperture remains in an identical position in the light beam.
The Jenway Genova and TrayCell cuvette are ideal partners for protein analysis and determining the concentration and purity of nucleic acids within a wide range of biological samples, in particular for applications including clinical biopsy samples where only very low volume samples are available.
In addition to providing the programs and calculations required by life scientists, the Genova offers all the flexibility of standard spectrophotometer functions, scanning mode, use with standard cuvettes, and the option of accessories such as sipper pumps and heated sample chambers.