Lysis Buffer for High Yield Enzymatic Shearing
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Porvair Sciences reports how its Chromatrap® Lysis buffer for enzymatic shearing provides users with a complete lysis buffer for the disruption of nuclear cell membranes with a milder detergent than that required for mechanical fragmentation.
Lysis plays a crucial role in enabling researchers to better explore and understand the underlying processes and mechanisms that drive biological organisms. Correct shearing is critical to the success of a ChIP assay. The buffer is compatible with all Porvair ChIP kits for enzymatic shearing from fixed cells or tissue.
Porvair's Chromatrap® Lysis buffers are formulated to ensure that the nuclear membrane in your samples is disrupted releasing a high yield of nuclear material for your research.
With the success of a ChIP assay being highly dependent on the quality of chromatin prepared, using a Chromatrap® Lysis buffer reagent to isolate chromatin from your sample can play an important role in the success of this process.