Morphology Preservation and Biomolecule Stabilization now Available in One Tissue System
Product News Jan 28, 2009
An integrated laboratory system for sample fixation, stabilization and purification is now available from PreAnalytiX GmbH. With the PAXgene Tissue System, researchers can for the first time perform histology, immunohistochemistry and stabilized RNA, miRNA, and DNA extractions from one sample without worrying about a compromised workflow.
Research laboratories working with human and animal tissue are faced with the challenge of conducting a variety of analyses on limited sample materials. The recognized standard for preserving tissue morphology is FFPE (formalin fixation/paraffin embedding). However, while this method provides excellent results for morphological studies, it does not preserve biomolecules. As a result, the DNA, RNA and miRNA molecules degrade, preventing the complete analysis required. This is a necessity for applications such as case-controlled and population-based clinical studies, facilitating biomarker discovery and, ultimately, the development of diagnostics and therapeutics.
The PAXgene Tissue system has two components; a tissue container and tissue kits. The dual-chambered container is pre-filled with two reagents: PAXgene Tissue Fix for fast penetration and fixation, and PAXgene Tissue Stabilizer for storage and transport.
The fixation process uses a standard histocasette with a fixative that is an alternative to formalin and is stopped by transferring the sample into the stabilizer. Fixation time is standardized at 2-4 hours. The sample is stable in the PAXgene Tissue Stabilizer for transport and storage prior to processing. Tissue can then be paraffin embedded and processed in pathology workflows. After tissue has been stabilized in PAXgene tissue containers (and optionally paraffin embedded), RNA, miRNA and DNA can be purified using the PAXgene tissue kits.
The PAXgene Tissue System from PreAnalytiX is the newest addition sold in QIAGEN’s suite of tissue management technologies. The suite includes integrated products that standardize all steps of the workflow, starting with archived or prospectively collected tissues and ending with data analysis. This ensures comparability and reproducibility of data from laboratory to laboratory.