New Application Notes for High Throughput PCR and SNP using the IDEX® Nanodrop™
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The first application note is entitled “Analysis on a LightCycler 480® and a Nanodrop Express Low-Volume Dispenser”, describes how the Nanodrop technology has been proven to be an efficient, cost effective tool in the preparation of PCR reactions for SNP detection in soybeans employing Melting Curve Analysis.
Melting Curve Analysis is a cost effective and efficient means for SNP detection and typically costs around $0.09 per 2 SNP with 3µl reactions. The combination of the Roche LightCycler® 480 and the Innovadyne Nanodrop Express is a powerful platform for high throughput SNP detection.
The second application note is entitled “Robotics Worklists for Creating 1536-Well Real Time PCR Assays” and focuses on how the utility of a worklist can enable the creation of custom plate layouts for higher density plates. High throughput PCR involves large numbers of samples and primers in all combinations and in replicates. CSV plate map worklists provide a logical set-up for these reactions and the Nanobuilder PatternViewer allows the contents to be viewed either by colour or text. This same information can be used to instruct the Nanodrop as to the required aspirate, dispense and wash steps required to create the destination plate.
Each application note is available as a downloadable PDF from the Gilson UK website at www.gilsonuk.com/nano/apps#pcr or on request by emailing info@gilsonuk.co.uk
