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New Applied Biosystems Reagent Kit Improves DNA Analysis
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Applied Biosystems, an Applera Corporation Business, has announced a new reagent kit designed to improve the workflow for DNA sequencing.
The BigDye® XTerminator™ Purification Kit addresses the often cumbersome purification or clean-up step in the Sanger Sequencing workflow to produce higher quality DNA sequence data with fewer steps than other purification methods.
Sanger sequencing is considered to be the gold standard method for DNA analysis. Part of the workflow includes a clean-up step to remove by-products of the sequencing reaction from the labeled DNA fragments.
The BigDye XTerminator Purification Kit is designed to shorten the amount of time and work required to perform the purification step, and to do so in a manner that is more reliable than other available methods.
Laragen, Inc., a service laboratory that performs DNA sequencing and analysis services for academic and commercial clients, is one of several organizations that tested the BigDye XTerminator Kit.
“We have tried virtually all of the commercially available methods for DNA purification, and the BigDye XTerminator Purification Kit has provided us with the highest quality results we’ve seen and with less manual labor than the other methods,” said Jin Li, Ph.D., vice president of Laragen. “Using this kit, we get very strong and even signals from beginning to end and longer sequencing reads, without dye blobs or lost samples.”
The BigDye XTerminator Purification Kit involves a simple 4-step protocol that provides dye blob removal, sample stability, and more reproducible, higher-quality results.
The kit consists of two reagents, the XTerminator™ Solution, which binds impurities but leaves DNA in solution, and the SAM™ Solution, an additive that enables complete removal of unincorporated terminators and stabilizes sequenced DNA fragments.
The product is designed to work in conjunction with the Applied Biosystems BigDye® Terminator sequencing chemistry, which is used to amplify and create fluorescent dye-labeled terminated fragments for automated sequencing analysis.
“The number of samples run for Sanger-based sequencing applications continues to increase worldwide,” said Kim Caple, Ph.D., senior director and general manager for Applied Biosystems’ genetic analysis business.
“One of our objectives at Applied Biosystems is to enable our customers to spend less time on sample preparation and the sequence clean-up process and instead enable them to focus on data generation and interpretation,” said Caple.
