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New Bio-Rad Technical Paper Compares Currently Available Affinity Columns
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Bio-Rad Laboratories, Inc. has announced the availability of a technical bulletin (number 5574A) that proves Bio-Rad’s Bio-Scale™ Mini Profinity™ cartridges offer comparable or better yield and purity to other commercially available prepacked affinity columns. The study was performed using Bio-Rad’s Profinia™ protein purification system.
Affinity-tagged purification of recombinant proteins is a common procedure performed in laboratories using glutathione-S-transferase (GST) and polyhistidine (His) affinity tags. The Profinia protein purification system simplifies the process by automating the purification and desalting of these tagged proteins in a single step, the company says.
Bio-Scale Mini Profinity chromatography cartridges are prepacked, disposable, and designed for use on chromatography instruments. The cartridges offer a number of advantages such as ease of use and reproducibility.
The Profinia system is a chromatography instrument that contains preprogrammed optimized methods for affinity purification of tagged proteins and antibodies resulting in increased efficiency and reproducibility in the laboratory. It takes 30 minutes to purify the protein of interest.
