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New Columns Accelerate LC/MS Analysis of Synthetic Oligonucleotides

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Phenomenex Inc. introduces Clarity® Oligo-MS columns for LC/MS characterization and quality control of synthetic RNA and DNA. Based on the company's core-shell particle technology, the new C18 columns deliver significantly shorter run times than traditional media, along with increased resolution and sensitivity. With the high resolving power of Clarity Oligo-MS, impurities in complex synthetic mixtures can be separated from the peak of interest in less than 10 minutes.

These new Oligo-MS columns, which are part of the Clarity BioSolutions portfolio, are packed with either 2.6µm or 1.7µm particles, enabling high efficiency on any HPLC or UHPLC platform.

The Clarity Oligo-MS 2.6µm columns operate at reduced backpressures compared to other oligonucleotide-specific columns, delivering UHPLC performance on any LC instrument, and methods are easily transferable. The Clarity Oligo-MS 1.7µm columns boost the performance of existing sub-2µm methods on high-pressure systems.

"LC/MS is the analysis technique of choice in synthetic oligo quality control as well as for the characterization of oligo-based therapeutics and their metabolites," explained Cathy Cordova, marketing manager for Phenomenex. "With our new core-shell particle technology, we bring significant improvements to this process in the areas of sample throughput and MS sensitivity."

According to Phenomenex, its core-shell technology enables high resolution and sensitivity over an extended linear velocity without generating excessive backpressure. These columns can provide roughly twice the efficiency of fully porous 3-micron columns and three times the efficiency of fully porous 5-micron columns, with lower limits of detection and quantitation.