New KAPA Library Quantification Kits Available from Anachem
Want to listen to this article for FREE?
Complete the form below to unlock access to ALL audio articles.
Read time: Less than a minute
The new KAPA Library Quantification Kits available from Anachem utilize the power of Quantitative PCR (qPCR) to provide user with an accurate and sensitive method for quantifying Next Generation Sequencing (NGS) libraries, the company has announced.
Standard methods for quantifying NGS libraries have a variety of disadvantages. Electrophoresis and spectrophotometry measure total nucleic acid concentrations, whereas optimal cluster density or template-to-bead ratio depend on the appropriate concentration of PCR-amplifiable DNA molecules. These methods also have low sensitivity, consuming nanograms of precious samples and are not suitable for high-throughput workflows.
qPCR, however, is said to be a highly sensitive approach which uses a minimal amount of material and eliminates the need for time-consuming and expensive titrations. It specifically quantifies only PCR-competent DNA molecules and due to its high sensitivity, allows for the accurate quantification of low concentration libraries and reduces cluster density variations. It can also be automated for high-throughput applications.
"Before qPCR was adopted for library quantification, cluster density was extremely variable. Implementation of the KAPA Library Quantification Kit into our sequencing workflows resulted in a significant reduction in variability across multiple libraries, negating the need for cluster amplification titration runs" - The Broad Institute, Cambridge, MA U.S.A.
The KAPA Library Quantification kits are optimized for the Illumina Genome Analyzer, Roche 454 Titanium series, and Roche 454 FLX series platforms and include defined, reliable DNA concentration standards and state-of-the-art qPCR reagents, containing a DNA polymerase engineered for SYBR® Green-based qPCR through a process of molecular evolution to provide a reliable method for quantifying PCR-amplifiable molecules in next-generation sequencing DNA libraries.
Standard methods for quantifying NGS libraries have a variety of disadvantages. Electrophoresis and spectrophotometry measure total nucleic acid concentrations, whereas optimal cluster density or template-to-bead ratio depend on the appropriate concentration of PCR-amplifiable DNA molecules. These methods also have low sensitivity, consuming nanograms of precious samples and are not suitable for high-throughput workflows.
qPCR, however, is said to be a highly sensitive approach which uses a minimal amount of material and eliminates the need for time-consuming and expensive titrations. It specifically quantifies only PCR-competent DNA molecules and due to its high sensitivity, allows for the accurate quantification of low concentration libraries and reduces cluster density variations. It can also be automated for high-throughput applications.
"Before qPCR was adopted for library quantification, cluster density was extremely variable. Implementation of the KAPA Library Quantification Kit into our sequencing workflows resulted in a significant reduction in variability across multiple libraries, negating the need for cluster amplification titration runs" - The Broad Institute, Cambridge, MA U.S.A.
The KAPA Library Quantification kits are optimized for the Illumina Genome Analyzer, Roche 454 Titanium series, and Roche 454 FLX series platforms and include defined, reliable DNA concentration standards and state-of-the-art qPCR reagents, containing a DNA polymerase engineered for SYBR® Green-based qPCR through a process of molecular evolution to provide a reliable method for quantifying PCR-amplifiable molecules in next-generation sequencing DNA libraries.
