New Kit Aims For Efficient Isolation of Circulating Cell Free DNA
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AMSBIO has expanded its range of cfPure™ Cell Free DNA Purification Kits for rapid and efficient isolation of circulating cell free DNA (cfDNA). A new cfPure™Max kit is now available for use on samples of 5mLs volume or greater.
AMSBIO cfPure® kits use silica-coated paramagnetic particles to purify cell free DNA from less than 1 mL to greater than 10 mL of serum or plasma. The buffers were developed to ensure efficient recovery of 100 bp – 500 bp DNA fragments in order to maximize recovery of cell free DNA. The recovered DNA is aimed to be suitable for a wide range of down-stream applications, such as bisulfite sequencing, NGS, and qPCR.
The cfPure® protocol is designed to be rapid, allowing users to process several samples in an hour or less and thus suitable for biomarker screening. Using proprietary silica-coated magnetic bead technology, cfPure® kits are designed to efficiently recover low molecular weight cell free DNA during isolation - a key success factor for successful analysis of cfDNA. Designed for scalability, cfPure® kits aim to enable the use of just the number of beads and amount of reagent that needed for cfDNA extraction.
The cfPure® kits allow you to use less magnetic bead solution if you are purifying small amounts of plasma – saving you money. Since cfDNA is typically found at very low concentrations and in a fragmented state, efficient DNA capture is vital to provide sensitive and reliable results.