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New Reagent from Applied Biosystems Accelerates Real-Time PCR

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Applied Biosystems has introduced a new master mix reagent designed to accelerate real-time PCR (polymerase chain reaction).

According to company, fast SYBR Green® Master Mix provides life scientists with a new choice in fast-enabled PCR chemistries that is expected to deliver results in less than half the time of standard SYBR green reagents1.

Fast SYBR Green Master Mix establishes Applied Biosystems as the only provider of complete workflow solutions for fast real-time PCR that include a portfolio of fast-enabled reagents, instruments, and other consumables.

Fast SYBR Green Master Mix was designed to accelerate the performance of real-time PCR while maintaining a high level of data quality by providing the complete mix of specialized chemistries for fast-enabled SYBR Green-based real-time PCR experiments.

SYBR Green dye is an easy to use nucleic acid labelling method for real-time PCR, which is commonly used in applications such as gene expression, chromatin immunoprecipitation (ChIP) analysis, and microarray validation, the company says.

Scientists at the Segal Cancer Center and the Lady Davis Institute at the Sir Mortimer B Davis Jewish General Hospital affiliated with McGill University are successfully using the Fast SYBR Green Master Mix as part of a fast real-time PCR workflow to study epigenetic and arsenic-based chemotherapy for leukaemia and solid tumours. These experiments generate gene expression and ChIP data that are important for advancing their research.

“The use of real-time PCR is vital to the research we are conducting and the more we can do to cost-effectively increase throughput, without compromising the quality of results, the better equipped we will be to advance our work,” said Dr Koren Mann, a project director at the Segal Cancer Center.

“Fast SYBR Green Master Mix provides a fast alternative with a familiar workflow that allows our researchers to easily integrate it into their existing real-time PCR applications. Fast SYBR Green Master Mix should allow us to achieve higher throughput while also maintaining the high level of sensitivity and specificity required for our gene expression and ChIP analysis research.”