We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
New UV Shadowing Application of G:BOX Image Analyser
Product News

New UV Shadowing Application of G:BOX Image Analyser

New UV Shadowing Application of G:BOX Image Analyser
Product News

New UV Shadowing Application of G:BOX Image Analyser


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "New UV Shadowing Application of G:BOX Image Analyser"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Syngene announces its G:BOX image system which is being used at the University of Southampton for a UV shadowing technique to detect unlabelled DNA, enabling scientists there to safely analyse a range of different oligonucleotides.

The G:BOX is being used by researchers in the School of Chemistry at the University of Southampton to visualise oligonucleotides in an acrylamide gel, placed on a silica-coated TLC plate. The G:BOX illuminates the oligonucleotides by shining UV light (254nm) on to the gel.

The oligonucleotides cast a shadow against the TLC plate’s fluorescent background, allowing visualization of the DNA bands with the G:BOX’s high resolution CCD camera. The image of the UV shadow is captured using the G:BOX’s capture software and the bands are then analyzed with GeneTools software to automatically calculate the size and molecular weight of the DNA.

Dr Afaf El-Sagheer, a Research Fellow in the School of Chemistry commented: “We are synthesizing different oligonucleotides using a variety of different organic reactions. We use UV shadowing to analyse them because it provides us with a clear impression of what is happening and is more convenient than using toxic stains like Ethidium bromide. We can detect as little as 0.1 OD260 of DNA and visualise DNA ranging from 2-300 bps using the G:BOX, which is sensitive enough for all our research needs.”

Laura Sullivan, Syngene’s Divisional Manager, added: “We are excited to see the G:BOX being used successfully for UV shadowing. The research at the University of Southampton demonstrates that the G:BOX offers an accurate method of detecting oligonucleotides or small sections of DNA without the need to use mutagenic Ethidium bromide, hazardous radiolabels or expensive non-toxic fluorescent dyes. For scientists with a limited consumables budget or those wanting to extract DNA from their gels, using the G:BOX image analysis system for UV shadowing provides the perfect solution.”

Advertisement