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NuGEN Announces Target Preparation Solution for Use with Agilent Dual-Mode Gene Expression Microarrays

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NuGEN Technologies, Inc. announced that it has made available a new amplification and labeling solution specifically for use with Agilent Dual-Mode Gene Expression Microarrays.

The solution and its simple protocol are suited for interrogation of a broad range of small and degraded clinical samples with as little as 500 picograms of input total RNA.

At the heart of the solution are NuGEN WT-Ovation™ Systems based on the Single Primer, Isothermal Amplification (SPIA®) technology used to generate cDNA from formalin-fixed, paraffin-embedded (FFPE) tissues, laser-captured, micro-dissected cells, fine needle aspirates, tissue biopsies, sorted cells, and other sample types that yield small or finite amounts of RNA.

The addition of this solution for Agilent Dual-Mode Gene Expression Microarrays completes the list of major expression analysis platforms with which the NuGEN Ovation® family of products is compatible. Others include the Affymetrix GeneChip® microarray platform, the Illumina Genome-Wide Expression BeadChip platform, and qPCR.

A simple, three-step protocol enables researchers to prepare targets in a single day from the most difficult samples:

• Choose the appropriate whole-transcript amplification system — the WT-Ovation™ FFPE System or the WT-Ovation Pico System.

• Amplify RNA to generate amplified cDNA.

• Label amplified cDNA and hybridize to Agilent Dual-Mode Gene Expression Microarrays.

The NuGEN Ovation family of RNA amplification and labeling products enables life scientists to conduct global gene expression profiling and signature discovery. Ovation Systems are platform independent, so researchers may conduct analyses using their preferred gene expression platform.