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NuGEN Launches The WT-Ovation™ Pico RNA Amplification System and the FL-Ovation™ cDNA Biotin Module V2
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NuGEN Technologies, Inc. has announced the launch of the WT-Ovation™ Pico RNA Amplification System and the FL-Ovation™ cDNA Biotin Module V2.
The latest amplification product in NuGEN's Ovation™ family, the WT-Ovation™ Pico System utilizes a Whole Transcriptome amplification approach, initiated both at the 3' and across the entire transcript, achieving an average of 10- to 15-thousand-fold linear amplification of mRNA with as little as 500 picograms of total RNA input.
Following a five-hour amplification process, micrograms of single-stranded cDNA product are generated and are ready for real-time quantitative PCR analysis, cDNA storage, or further processing for fragmentation and labeling.
Using NuGEN's FL-Ovation™ cDNA Biotin Module V2, the amplified cDNA can be fragmented and labeled for GeneChip® array analysis in two hours without the need for further purification.
This enables investigators to prepare cDNA target from picogram levels of total RNA and proceed to array hybridization in one day.
"The combination of a 3' and whole transcriptome amplification approach utilized in the WT-Ovation™ Pico System enables amplification of compromised and non-polyA RNA samples," said Garry Miyada, vice president of development at NuGEN.
"In addition to providing flexibility in qPCR assay design, this approach provides opportunities to study splice variants, examine archived or degraded samples common to cancer studies, investigate prokaryotic samples, and still be able to bank and store the stable cDNA amplification products of precious RNA samples and clinical specimens."
"The WT-Ovation™ Pico System with 500 picogram RNA input requirements demonstrates the power of our technology," said Sue Pandey, vice president of commercial operations for NuGEN.
"The approach opens the door for gene expression analysis of large sample sets comprised of very small and complex specimens, previously beyond the reach of investigators."
"Like other Ovation™ System products, the simple, automation-friendly process in both new products is specifically suited to the high throughput target preparation and sample processing requirements of today's discovery and clinical research laboratories."
