Optimized ChIP Protocols for qPCR and Sequencing
Chromatrap® has announced a new web page that brings together protocols developed using their proprietary solid state chromatin immunoprecipitation (ChIP) technology.
The new web page provides scientists with optimized ChIP methodologies for qPCR, high throughput qPCR, sequencing, high throughput sequencing and enzymatic chromatin shearing using Chromatrap® kits.
Chromatrap® is a technology that offers a quicker, easier and more efficient way of undertaking ChIP assays. Chromatrap® kits for qPCR use revolutionary Chromatrap® Protein-A or Protein-G spin columns which contain discs of an inert, porous polymer to which protein A/G has been covalently attached.
During an assay, the chromatin/antibody complex is retained by the disc. Flushing with three buffers and an elution step are all that is required to obtain the DNA fragments of interest. Operating without the use of magnetic or agarose beads all but eliminates non-specific background and drastically reduces manual handling error and sample loss.
Optimized elution buffer chemistry allows samples to be analyzed directly in qPCR without the need for DNA clean up, further reducing sample loss.