Pluripotent Stem Cell Line Assessment qPCR Kit
Product News May 23, 2011
AMSBIO has announced the launch of the PluriPCR™ Kit, a new benchmark to revolutionize the definition of stem cell pluripotency.
The PluriPCR kit has been designed as a quantitative, easy to use, and reliable assay of five genes strongly specific to pluripotency. These genes: Oct-3/4, Nanog, DNMT3b, Dppa4, and Rex1 are expressed by human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC); all are sharply down-regulated during differentiation.
For the first time ever stem cell scientists are now able to accurately and unambiguously define the pluripotency of a cell line using the PluriPCR Kit. Both simple and easy to use with existing real time quantitative PCR equipment.
PluriPCR uses five key genes, in combination with a unique normalization method, which gives a reliable and quantitative readout for the pluripotency of a cell line.
This kit is ideally suited for all aspects of pluripotent stem cell research - measure if culture conditions are resulting in the loss of pluripotency, to assess successful nuclear reprogramming of iPS cells and even as a release assay in the manufacture and clinical development of pluripotent stem cells.
The PluriPCR kit is fully optimized for routine quality control using either two-step or one-step Real-time Quantitative PCR (qRT-PCR) methods. The assay kit combines multiple pluripotency and housekeeping markers and an analysis tool to assess the pluripotency of your cell line.
The resultant PluriPCR Index is a simple method to understand if your pluripotent stem cells are differentiating.