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Pluripotent Stem Cell Line Assessment qPCR Kit
Product News

Pluripotent Stem Cell Line Assessment qPCR Kit

Pluripotent Stem Cell Line Assessment qPCR Kit
Product News

Pluripotent Stem Cell Line Assessment qPCR Kit

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AMSBIO has announced the launch of the PluriPCR™ Kit, a new benchmark to revolutionize the definition of stem cell pluripotency.

The PluriPCR kit has been designed as a quantitative, easy to use, and reliable assay of five genes strongly specific to pluripotency. These genes: Oct-3/4, Nanog, DNMT3b, Dppa4, and Rex1 are expressed by human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC); all are sharply down-regulated during differentiation.

For the first time ever stem cell scientists are now able to accurately and unambiguously define the pluripotency of a cell line using the PluriPCR Kit. Both simple and easy to use with existing real time quantitative PCR equipment.

PluriPCR uses five key genes, in combination with a unique normalization method, which gives a reliable and quantitative readout for the pluripotency of a cell line.

This kit is ideally suited for all aspects of pluripotent stem cell research - measure if culture conditions are resulting in the loss of pluripotency, to assess successful nuclear reprogramming of iPS cells and even as a release assay in the manufacture and clinical development of pluripotent stem cells.

The PluriPCR kit is fully optimized for routine quality control using either two-step or one-step Real-time Quantitative PCR (qRT-PCR) methods. The assay kit combines multiple pluripotency and housekeeping markers and an analysis tool to assess the pluripotency of your cell line.

The resultant PluriPCR Index is a simple method to understand if your pluripotent stem cells are differentiating.