Pre-designed CompoZr® Knockout Zinc Finger Nucleases for Entire Human Genome
Product News Feb 28, 2011
The new offer can generate permanent and heritable gene knockouts in human cell lines within weeks.
Currently, few laboratories create gene knockouts in human cell lines because the inefficiencies of the natural cellular homologous recombination system and the investment of time and labor required to screen the resulting cells. In contrast, a wealth of peer-reviewed literature has shown that CompoZr ZFNs efficiently generate human cell lines with precise and stable gene knockouts in matter of weeks.1
“Sigma Life Science has worked diligently to develop predesigned CompoZr Knockout ZFNs for each gene in the human genome to make this ground breaking technology more readily available to the research community,” said David Smoller, Chief Scientific Officer of Sigma Life Science. “Researchers can select a ZFN from our library and swiftly knockout their target gene in any human cell line.”
In this new offering, Sigma has designed CompoZr ZFNs that will produce a knockout in each gene by inducing a double-strand break at a defined site within the gene’s first three exons. This double strand break stimulates the cell's natural DNA repair pathways, resulting in a permanent, site-specific deletion or mutation that disrupts the gene’s function. Robust and on-target gene knockout using CompoZr Knockout ZFNs is guaranteed by functional validation prior to shipment and unique enhancements to the DNA-recognition and cleavage ZFN subunits.