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Preparing High Quality Fragmented Chromatin for ChIP Analysis
The Chromatrap® Enzymatic Shearing Kit provides an excellent method for the preparation of high quality, ideally fragmented chromatin for ChIP analysis.
The success of a ChIP assay is highly dependent on the quality of chromatin prepared. The Enzymatic shearing kit provides buffers to optimise the shearing conditions of your chromatin by enzymatic digestion.
Chromatin prepared using this kit demonstrates excellent enrichment, independent of starting cell number. With its quick and simple protocol the Chromatrap® Enzymatic Shearing Kit is the perfect cost-effective alternative to sonication.
The Enzymatic Shearing Kit from Chromatrap contains all the necessary reagents and buffers for up to 10 chromatin preparations. This generates enough chromatin to perform up to 24 ChIPs using a standard Chromatrap® ChIP spin column kit or up to 96 IPs using the Chromatrap® 96 HT microplate kit.
Source:
Story from Chromatrap/Porvair Sciences. Please note: The content above may have been edited to ensure it is in keeping with Technology Networks’ style and length guidelines.
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