We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
PrimePCR™ Assays Used to Create qPCR Reference Data for FDA-Led Sequencing Project
Product News

PrimePCR™ Assays Used to Create qPCR Reference Data for FDA-Led Sequencing Project

PrimePCR™ Assays Used to Create qPCR Reference Data for FDA-Led Sequencing Project
Product News

PrimePCR™ Assays Used to Create qPCR Reference Data for FDA-Led Sequencing Project


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "PrimePCR™ Assays Used to Create qPCR Reference Data for FDA-Led Sequencing Project"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

This FDA-led Sequencing Quality Control (SEQC) study compared RNA-seq data generated using different NGS platforms to benchmark their performance. The authors compared different NGS platforms and how they performed on well-characterized RNA samples. They also compared those data to microarray and qPCR data.

Biogazelle used almost 21,000 PrimePCR Assays to assess the expression levels of protein-coding genes in four samples from MAQC-I, a previous FDA-led study to compare different microarray technologies. In total, Biogazelle performed more than 80,000 qPCR reactions. These reference data matched well with RNA-seq results.

“Our motivation to participate in the SEQC study was to add a valuable reference for evaluating the results generated by RNA sequencing,” said Jan Hellemans, CEO and cofounder of Biogazelle. “Bio-Rad’s human PrimePCR Assays are fully wet-lab validated, making them ideally suited for benchmarking.”

JanHellemans.jpg

Jan Hellemans, CEO and cofounder of Biogazelle.

Bio-Rad and Biogazelle partnered to develop PrimePCR Assays in 2012, seeking to achieve a level of validation that goes beyond industry standards and common research practices. The raw validation data that come with each assay ensure studies are predictable and controlled. Researchers can also use PrimePCR Assays to follow qPCR best practices known as MIQE (minimum information for publication of quantitative real-time PCR experiments).

Advertisement