Purification of miRNA and Total RNA from FFPE Tissue
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QIAGEN has expanded its range of technologies for miRNA research with the launch of the miRNeasy FFPE Kit. The kit enables purification of total RNA that includes miRNA and other small RNA from approximately 18 nucleotides upwards, from formalin-fixed, paraffin-embedded (FFPE) tissue sections including laser capture microscopy samples. The innovative procedure reverses formalin crosslinking, which would otherwise block downstream applications.
Fixing tissues with formalin leads to RNA–RNA and RNA–protein crosslinking, which impairs RNA performance in downstream applications. The miRNeasy FFPE Kit can provide special lysis and incubation conditions to reverse formalin crosslinking of RNA.
In addition, the specially developed lysis buffer releases RNA from tissue sections while avoiding further RNA degradation. The kit also uses gDNA Eliminator spin columns for removal of genomic DNA contamination. Optimized binding conditions allow purification of all usable RNA from approximately 18 nucleotides upwards. The purified RNA delivers maximal performance in a range of applications, including real-time PCR.