QIAGEN Launches Products for Gene Expression Analysis
Product News Jun 23, 2008
RNA purification and messenger RNA (mRNA) quantification can be done with less sample material using three new products introduced by QIAGEN this month.
The RNeasy® Plus 96 Kit is a 96-well RNA purification kit that does not require DNase digestion, even for applications particularly sensitive to minute amounts of DNA, the company says.
According to QIAGEN, the QuantiFast® Multiplex PCR Kit is the first to integrate multiplex technology with fast-cycling capabilities, making real-time PCR and two-step RT-PCR up to two times faster to complete.
In another first, the AllPrep® DNA/RNA 96 Kit is the only kit that can simultaneously purify genomic DNA and total RNA from the same sample using a 96-well format, saving time by as much as 50 percent, QIAGEN claims.
Gene expression and function analyses are critical to understanding the role of various genes in the development of serious diseases such as cancer. The two most important steps for an accurate analysis of gene expression are RNA purification and quantification of mRNA.
“As researchers are facing intense competition to publish and win funding — pushing them to work faster, they are seeking solutions that not only offer high sensitivity and reproducibility, but also significant time savings and conservation of precious samples,” said Dr. Annette Tietze, Global Product Manager at QIAGEN.
“That is why technologies that allow simultaneous purification of DNA and RNA, simple workflows, standardized procedures and ready-to-use kits are in demand. QIAGEN’s new products for RNA purification and mRNA quantification meet these needs.”
The QuantiFast Multiplex PCR Kit uses real-time PCR to analyze up to four targets at once by integrating multiplex technology with a fast-cycling capability. The result is significant reduction of PCR run times of up to 50 percent and, as well as the ability to save precious sample material.
In addition, the multiplex technology allows reliability in results by simultaneously detecting an endogenous control gene in the sample of interest. The kit also offers a specifically developed master mix and a universal protocol that can be used on all available real-time cyclers – both standard and fast – thus eliminating the need for optimization of reaction and cycling conditions.
The AllPrep DNA/RNA 96 Kit allows simultaneous high-throughput purification of genomic DNA and total RNA using the same sample and a 96-well format. The kit cuts processing time by up to 50 percent and is suitable for purification of high-quality DNA and RNA from cultured cells and easy-to-lyse tissues.