Qiagen Launches QuantiFast Kits for Fast PCR on Real-Time Cycler
Product News Jan 30, 2007
QIAGEN has introduced QuantiFast™ Kits which are designed to deliver fast results in real-time PCR. In addition, no PCR optimization is required. Kits are available for SYBR® Green or probe-based detection in PCR, two-step RT-PCR, and one-step RT-PCR.
A specially developed PCR buffer containing the patent-pending additive Q-Bond can reduce denaturation, annealing, and extension times.
The buffer also contains HotStarTaq® Plus DNA polymerase, which requires only 3 or 5 minutes at 95ºC for activation. For one-step RT-PCR, the optimized QuantiFast RT Mix can enable cDNA synthesis in just 10 minutes.
Accurate quantification of even low copy numbers are achieved with QuantiFast Kits. A balanced combination of KCl and NH4Cl in the fast PCR buffer promotes specific primer annealing, which can enable high PCR specificity and sensitivity. In addition, HotStarTaq Plus DNA polymerase provides a stringent hot start, preventing the formation of nonspecific products.