QIAGEN Launches QuantiFast® Multiplex RT-PCR Kits and Rotor-Gene® Multiplex RT-PCR Kits
Product News May 14, 2009
QIAGEN launches QuantiFast® Multiplex RT-PCR Kits and Rotor-Gene® Multiplex RT-PCR Kits. The QuantiFast Kits are the first-to-market in multiplex real-time RT-PCR without requiring optimization on both standard and fast real-time cyclers for one-step applications.
The Rotor-Gene Kit allows detection of up to four targets per tube and includes optimized protocols for precise results on the Rotor-Gene Q. With the introduction of these kits, QIAGEN becomes the first vendor to be able to offer customers a complete portfolio for 1-step and 2-step RT-PCR.
Gene expression and function (GEF) analysis plays an important role in areas such as cancer research, enabling biomarker identification, target validation, and drug development. Of importance to researchers is the ability to achieve accurate real-time PCR with minimum time and effort. These real-time PCR technologies provide speed and accuracy not only on fast cyclers, but also on standard cyclers. For applications such as gene silencing by siRNAs, the entire real-time PCR workflow can be streamlined by eliminating the need for RNA purification and allowing analysis of multiple targets in one tube.
Another key component in the gene expression workflow is the new TissueLyser LT. This instrument uses bead mill technology to break-up tissue and provide fast access to DNA, RNA, and proteins. This bead mill can process up to 12 samples, fresh or frozen, in parallel using the carousel adaptor. The adaptor is coolable for sensitive applications such as plant disruption, preventing degradation. The 12 sample format has an additional benefit for current QIAGEN automation users - integration of sample disruption with other QIAGEN automation such as the QIAcube® for purification and QIAxcel® for analysis.
In addition to the newest offerings for RT-PCR analysis, QIAGEN today announces the launch of AllStars Control siRNA for transfection optimization in mouse and rat cells. AllStars Control siRNA enables transfection optimization with phenotypic control siRNA in virtually all mouse and rat cell types. Control siRNA is used for transfection optimization and as a positive control in high- throughput screening in mouse and rat cells.