QIAGEN Launches REPLI-g® FFPE Kit
Product News Jan 22, 2008
QIAGEN launches a new product that supports the genomic analysis of fixed tissue samples. The REPLI-g® FFPE Kit, the latest development in QIAGEN’s whole genome amplification portfolio is designed to eliminate problems associated with DNA fragmentation and damage caused by formalin fixation.
The kit uses a DNA processing reaction, allowing the advantages of multiple displacement amplification (MDA), a key technology in the amplification of genomic DNA, to be extended to highly degraded DNA samples derived from FFPE tissue.
Isolation of genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue is often difficult due to the low amount of DNA available.
The REPLI-g® FFPE Kit allows amplification of sample material while maintaining locus representation, enabling unlimited downstream analyses to be performed. Highly uniform whole genome amplification directly from formalin-fixed, paraffin-embedded (FFPE) tissue can now be performed without the need for prior DNA isolation.
The REPLI-g® FFPE Kit can provide amplification resulting in scalable and standardized DNA yields. Following lysis of the tissue section, the DNA is processed using buffers and enzymes that ligate fragmented DNA. The long DNA strands created by the ligation reaction are amplified using proven REPLI-g technology.
A two-hour amplification reaction typically yields 10 µg DNA, whereas 40 µg can be obtained after eight hours incubation. Once amplified, the DNA is suitable for immediate use in most downstream genotyping assays without further purification.