Stable Cell Line Generation Using Lentivirus
Product News May 29, 2012
AMSBIO has announced a fast service for custom generation of high quality, affordable over-expressing or knockdown stable cell lines.
AMSBIO's custom generation of stable cell lines includes cloning the gene or shRNA of interest into a safe lentiviral vector, generating the lentivirus and transducing into the cell line of users choice.
The service allows users to choose a constitutive or inducible promoter, a tag, an antibiotic selection marker and a fluorescent marker.
The ease of providing users target templates or choosing one from AMSBIO's cDNA collection ensures rapid turnaround.
With many years of experience the service can also provide mutagenesis and other special requests.
The stably transduced, high expression cells are then selected, target integration is validated by genomic PCR, and the high-expression clone by Western Blot.
Each customer receives two cryogenic preserved vials of stable cells and a final report.
Working closely with customers - AMSBIO's handles each stage of the process to ensure the delivery of high quality cells that are ready to use. AMSBIO's unique technology ensures a turnaround time as fast as 2 months for each cell line creation.
The AMSBIO lentiviral system is a gene delivery tool for gene expression or knockdown.
Lentivirus can effectively transduce both dividing and non-dividing mammalian cells, and integrate into the host genome, allowing stable long-term, high-level gene expression both in vivo and in vitro.
AMSBIO's stable cell line generation service only use safe (self-inactivating) lentiviral particles to deliver users gene into a wide range of cell lines including primary and stem cells.
Lentiviruses have been used to successfully generate a line of DNA repair knock-down cell lines that AMSBIO offers off the shelf from its product range.