Tech Note Examines How to Efficiently Transfect Mouse and Human Embryonic Stem Cells
Product News Dec 15, 2009
Bio-Rad Laboratories, Inc. has announced the availability of a technical note titled, “Transfection of Mouse and Human Embryonic Stem cells by Electroporation Using the Gene Pulser MXcell™ System” written by Eva Zsigmond of the University of Texas Health Science Center.
The technical note (tech note 5904) examines ways to optimize electroporation parameters for the efficient transfer of DNA into mouse embryonic stem cells and human embryonic stem cells that have been grown under colony-forming and feeder-free conditions.
Historically, electroporation has been the method of choice for introducing genes into mouse embryonic stem cells. However, compared to mouse embryonic stem cells, human embryonic stem cells have shown low cell viability and transfection efficiencies after electroporation.
Publications on electroporation-based gene targeting for human embryonic stem cells are scarce. It is important to use a delivery technique such as electroporation that doesn’t affect the stem cell phenotype.
A key advantage of Bio-Rad’s Gene Pulser MXcell electroporation system over other systems is that in addition to preset protocols, every critical parameter is programmable-from voltage to number of pulses-enabling researchers to find the ideal set of conditions for their specific cells, including difficult-to-transfect primary and stem cells.
The Gene Pulser MXcell electroporation system’s “open” architecture permitted Zsigmond’s research team to determine the optimum protocols for efficiently electroporating both mouse and human embryonic stem cells while optimizing cell viability. The full technology note is available through Bio-Rad’s website.