Techne TC-PLUS Thermal Cycler for new Fast PCR Protocols
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Bibby Scientific announces that the Techne TC-Plus thermal cycler has been shown to contribute to savings in PCR reaction times by using faster ramp rates, modified programs and modified reaction mixes.
According to the company, time savings are an advantage in many research labs and the Techne TC-Plus is designed for use when optimizing for fast PCR protocols. This thermal cycler offers flexible programming and rapid block ramp rates of up to 5ºC per second.
Comparing the same PCR program on the TC-Plus and the slower TC-512 gave a time saving of around 30 minutes simply by using faster ramping (1 hour compared with 1 hour 30 minutes). There were no appreciable differences in yield or sensitivity between the two thermal cyclers.
Another way to shorten run times is to modify the standard protocol by reducing the number of steps and/or hold times of individual steps. A temperature gradient optimization experiment changing from three to two steps using the TC-Plus saved 22 minutes (52 minutes run time for the two-step protocol; 1 hour 14 minutes for three steps). There was a decrease in the amount of 400bp fragments, but no noticeable difference in yield of the 100bp PCR product, the Company claims.
