Thermo Fisher Scientific Offers ABsolute Blue QPCR Master Mixes
Product News Feb 27, 2008
Thermo Fisher Scientific Inc. specializes in the provision of optimized consumables for reproducible, sensitive and accurate QPCR assays, including Thermo Scientific ABsolute™ Blue QPCR Master Mixes. This colored range of high performance QPCR master mixes is specially formulated to achieve sensitive and consistent data across each of the major QPCR platforms whilst enhancing the contrast between reagent and plastic to ensure accurate dispensing, the company says.
Evangelia Siaterli, contract services manager at Oxford Expression Technologies Ltd., UK, comments, “Now that I’m using the ABsolute Blue Master Mix, I get consistent and reproducible data across all my QPCR runs, together with improved reaction sensitivity. The fact that it is blue makes it different from other mixes, as it improves the contrast between the reagents and the plate. This allows me to visualize accurate dispensing of the master mix in each well, which is extremely useful, especially when dealing with a large number of reactions."
The combination of Thermo Scientific Thermo-Start™, a chemically-modified hot start Taq DNA polymerase, and the incorporation of dTTP instead of dUTP in ABsolute Blue Master Mixes ensures increased reaction sensitivity and specificity.
In trials, ABsolute Blue produced higher end-point values and lower Ct values when compared to competitor QPCR master mixes. In addition, the inclusion of an inert blue dye helps users to easily verify accurate dispensing, thus increasing confidence in results.
There is an optimized ABsolute Blue Master Mix available for all major QPCR platforms and detection chemistries. These mixes belong to the Thermo Scientific range of high performance QPCR consumables, including specialized QPCR plates and seals, which have been developed to ensure optimal fluorescence detection.