Thermo Fisher Scientific Offers Extensor Long PCR Enzyme for Amplification of Long DNA Fragments

Thermo Fisher Scientific Inc. has introduced Thermo Scientific Extensor Long PCR Enzyme Mix for the amplification of long DNA fragments.

According to Thermo Fisher Scientific, the Extensor Long PCR Enzyme Mix offers up to six times the fidelity and double the yield of regular Taq DNA polymerase in the amplification of long DNA fragments. This makes it ideal for applications such as cloning and mutagenesis or for the amplification of difficult and GC-rich fragments.

This blend contains ThermoPrime Taq DNA Polymerase and a proprietary, thermostable proofreading enzyme which act synergistically to ensure reliable amplification of DNA templates up to and in excess of 20kb.

The Extensor Enzyme Mix is available with two different buffers: one to optimize the amplification of fragments up to 12kb and the other for amplicons longer than 12kb or problematic PCR (such as GC-rich fragments).

Each combination is also available pre-blended in a convenient master mix format to simplify PCR set-up and reduce the risks of contamination or reagent handling errors. These master mixes can be stored at 4º Celsius, eliminating the need for freeze/thaw cycles that may reduce the activity of the enzymes.

Extensor Long PCR Master Mixes are also available with ReddyMix™, which incorporates a red tracker dye and precipitant to allow direct loading of the PCR product onto gel for post-PCR analysis.