Thermo Scientific Introduces New Single-Step In Vitro Translation Kits Based on Human Cell Lysates
Thermo Fisher Scientific has introduced two new kits that enable the in vitro expression of full-length proteins from mRNA or DNA templates with yields of up to 750 micrograms per milliliter of reaction.
The expressed proteins by either the Thermo Scientific 1-Step Human Coupled IVT Kit for DNA or the 1-Step Human IVT Kit for RNA can be used to study protein interactions, to perform mutational analysis or for structural studies.
The 1-Step Human IVT Kits rely on the unique HeLa cell lysate that contains all the machinery for in vitro protein expression.
While 1-Step Human Coupled IVT reactions are complete in 90 minutes, reactions can be extended for up to six hours for higher protein yields.
These kits can produce proteins greater than 250 kilodaltons in size and can express functional ezymes, phosphoproteins, glycoproteins and membrane proteins.
In addition to the 1-Step Human IVT Kits, 10 new expression vectors optimized for use with these lysates are now available. The vectors offer the choice of different protein purification tags.
All vectors contain the EncephaloMyoCarditis Virus (EMCV) Internal Ribosome Entry Site (IRES) that is critical for high-level translation of mRNAs.
A custom service to clone target sequences into these vectors is also available.