TransPlex™ Whole Transcriptome Amplification Kit for Improved Expression Profiling
Product News Sep 09, 2005
Rubicon Genomics, Inc. has announced the launch of its TransPlex™ whole transcriptome amplification (WTA) kit for amplification of nanogram quantities of RNA from fresh and formalin-fixed tissue.
According to Rubicon, in less than four hours, 10 nanograms of RNA can be amplified into 10 micrograms of stable cDNA product without detectable 3' bias or background.
TransPlex kits convert total RNA into in vitro cDNA libraries of short, overlapping amplimers that represent the entire transcriptome of any animal, plant, or microorganism.
These molecular libraries are amplified by universal-primer PCR, which has the advantage of both exponential amplification kinetics and linear product representation.
TransPlex shows little gene- or exon-bias for transcripts up to 10 kb in length, even after extensive RNA degradation.
"We continue to execute our strategy to become the industry standard for managing genetic resources," said Fred G. Beyerlein, president and chief executive officer, Rubicon Genomics, Inc.
"With the roll out of our TransPlex kits we are looking to reach another tier of our target market and put our products in the hands of the users.”
"The knowledge and experience we have gained in our service business, in cooperation with our clients, has enabled us to put a superior product on the market."
RNA has been amplified from formalin-fixed biopsies and shown to perform equally well on the Affymetrix U133 or X3P GeneChip® -- giving excellent performance with 95 percent correlation with IHC and pathology results, independent of the age of the biopsies or condition of the RNA.
Q-PCR has been used to quantify the faithful representation of expressed sequences from both unfixed and fixed materials isolated conventionally or by LCM.
TransPlex kits contain proprietary reagents and protocol sufficient for 24 microarray hybridizations.
The protocol is faster, reproducible, and less labor intensive than alternative RNA amplification methods, and is scaleable from single tubes to 384-well plates.
Once amplified, the TransPlex cDNA is stable and can be reamplified even after years of storage.