Troubleshooting and Optimizing Mutagenesis

As part of the new, free-to-download IDT Mutagenesis Applications Guide, an extensive troubleshooting section makes it possible to optimize your mutagenesis experiments for the best possible resulting data. Everything from primer design, reaction setup, ligation and transformation efficiency, to specific reaction components and PCR parameters, are covered. The guide provides a useful reference tool for performing site-directed or random mutagenesis.

The Mutagenesis Applications Guide also describes IDT’s range of Ultramer™ Oligonucleotides, which can simplify mutagenesis experiments. These high fidelity oligonucleotides provide greater experimental flexibility than standard oligonucleotides due to their lengththey are available up to 200 bases.