TwistDx Launches World’s First DNA Amplification Kit Based on Recombinase Polymerase Amplification
Product News Jul 21, 2009
TwistDx, a developer of point-of-use nucleic acid diagnostics, announced the launch of its TwistAmp™ product range, which is said to be the world’s first DNA amplification products based on Recombinase Polymerase Amplification (RPA).
Concurrently, TwistDx also launched the Twista™ portable, real-time fluorometer RPA reader. RPA is a proprietary technique, employing recombinase enzymes, used to amplify nucleic acids in any biological sample, with high analytical sensitivity and specificity.
According to Company, each of the four currently available TwistAmp™ kits offers scientists the ability to detect DNA molecules in a mixed sample within 10-15 minutes, less than half the time of most PCR systems.
Moreover, RPA operates at low, constant temperatures and does not even require initial melting of the sample DNA, opening the way to broader access to end-users without reliance on expensive thermocyclers.
RPA has also been shown to be highly resistant to crude samples in comparison to PCR suggesting applications in on-the-spot field testing requiring no nucleic acid extraction.
The Twista™ portable real-time fluorometer is a customized platform containing proprietary fluorescent probe systems developed by the company to monitor RPA reactions. Although seamlessly integrated with the Twista™ device, TwistAmp™ kits can be used in combination with various, widely used assay platforms, such as conventional analysis tools, dipsticks, as well as broadly available fluorescence plate readers and real-time PCR machines.