Versatile Microfluidics Chip Technology Extended to Target Selection Applications
Product News Mar 02, 2009
LC Sciences has announced the availability of a custom target enrichment service for next-generation sequencing applications. LC Sciences now provides a service for Target-Specific Selection of a defined genomic region or RNA sequences. This new service enables researchers to take full advantage of new “next-generation” high throughput sequencing technologies.
LC Sciences µParaflo® technology’s combination of advanced microfluidics and synthesis chemistry provides a robust synthesis process which is necessary to achieve high quality selection and the flexibility to handle almost any target selection project.
The technology has been used to produce custom sequence microarrays and custom designed oligonucleotide mixture libraries (OligoMix®) since 2003 and is now particularly well suited to providing target selection solutions.
LC Sciences’ microarray based approach utilizes thousands of custom oligonucleotide sequences which are synthesized in situ on a programmable high density microfluidics chip as capture probes designed to target specific regions of interest in any genome (or transcriptome).
Samples are hybridized to the chip, undesired sequences are washed off and the captured target sequences are recovered by eluting them from the chip. The selected target sequences are ready for high-throughput sequencing or further processing.
When target selection in-solution makes sense, this flexible chemistry can directly synthesize biotinylated or phosphorylated oligonucleotides that are designed to target specific genomic/sequence regions of interest. After hybridization with a sample, magnetic beads are added and the capture probes are affinity linked to the beads. The captured target sequences are separated from other sequences by washing the beads after which the target sequences can be recovered. Alternatively, the oligonucleotides (OligoMix®) may be immobilized on beads prior to target selection, again depending on the needs of the particular project.
LC Sciences currently offers its Target-Specific Selection application as a service, as it has successfully done with other applications of the µParaflo® technology such as miRNA profiling, small RNA discovery, kinase profiling and epitope mapping with peptide arrays, and others.