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Whole-Transcriptome Expression Analysis From A Single Cell

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Based on the company’s proven amplification technology, the WT-Ovation™ One-Direct RNA Amplification System uses a simple, three-step process to prepare samples directly from cell lysate for analysis using microarrays or qPCR.

The WT-Ovation One-Direct RNA Amplification System makes possible microarray analysis from one cell or just a few cells, while eliminating costly, time-consuming RNA purification.

"The One-Direct System offers a significant advancement for scientists exploring biology at the single cell level," explained Dr. Yan Zhang, NuGEN vice president of marketing. "Because biological samples are composed of heterogeneous cell populations, elucidating the contribution of each individual cell in such a complex matrix requires the granularity of a true single-cell interrogation methodology."

Global, Whole-transcriptome Gene Expression Analysis
This breakthrough is important to academic laboratories, pharmaceutical companies, clinics, and hospitals focused on basic discovery and disease research. It will enable researchers to develop more effective diagnostic tools and more targeted drug therapies.

"The ability to assay complex gene expression patterns in single cells is a transformative step in systems biology," noted Dr. W. Nicholas Haining, assistant professor of pediatrics, department of pediatric oncology, Dana Farber Cancer Institute. "One can imagine broad-ranging applications of the NuGEN WT-Ovation One-Direct System in immunology, neurobiology, hematopoiesis, and cancer stem cell biology."