Retain Rare RNA Transcripts With New rRNA Depletion Kit

SEQuoia RiboDepletion Kit is a stand-alone, post-library depletion kit that eliminates fragments derived from cytoplasmic ribosomal RNA (rRNA) and mitochondrial ribosomal RNA (mt rRNA) sequences from an RNA-Seq library. The ribosomal RNA is abundant, constituting 80–90% of total RNA. Efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples.

Depletion of rRNA from total RNA before library construction can result in the loss of rare or low-abundance transcripts. SEQuoia RiboDepletion Kit depletes cDNA library fragments that were derived from rRNA post-library preparation, exhibiting superior performance in profiling the whole transcriptome, retaining and capturing small RNAs while minimizing the loss of rare transcripts and RNA from limited or degraded samples. This stand-alone ribodepletion kit is optimized to work with a broad input range (0.1–20 ng of RNA-Seq library) and is compatible with most available library prep kits. With the innovative post-library depletion technology, multiple pre-indexed libraries can be pooled and depleted in a single reaction, maximizing the depletion efficiency and saving significant time and cost. The streamline workflow can be finished within 2 hours.

About Bio-Rad

For over six decades, Bio-Rad has served life science research and clinical diagnostics customers worldwide through its global network of operations. Bio-Rad is internationally renowned among academic institutions, pharmaceutical companies and major research institutions for its commitment to quality and customer service.

Interested in seeing more content from Bio-Rad?

Discover all their latest news, webinars, products and more by clicking the link below.

Chosen for You