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Reveal More With the PacBio Revio System’s Exceptionally Accurate Long-Read Sequencing

Reveal More With the PacBio Revio System’s Exceptionally Accurate Long-Read Sequencing


The Revio system adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

Accuracy—The Revio system provides reliable answers through exceptional accuracy, with 90% of bases ≥Q30 and median read accuracy ≥Q30.


Throughput—With a high-density, 25 million ZMW SMRT Cell, up to 4 SMRT Cells in parallel, and 24-hour run times, the Revio system delivers 360 Gb of HiFi reads per day, equivalent to 1,300 human whole genomes per year.


Affordability—HiFi sequencing provides small variants, structural variants, repeat expansions, methylation, and haplotype phasing from a single library and sequencing run for a complete $1,000 phased genome.


Efficiency—Operating the Revio system is simple and uses 50% fewer consumables, thanks to a combined reagent and sample plate, rapid run setup, flexibility to queue runs while sequencing is in progress, and no external nitrogen supply.


Methylation calling—Advanced algorithms on the Revio system deliver exceptional read accuracy plus methylation calling in every run. Its optimized file formats reduce data storage needs through quality value binning and smart read ordering.


Product Specifications
Read length 15-18 kb
Read accuracy 99.95% (Q33)
Run time 24 hours
Yield 90 Gb
Variant calling SNVs, SVs, and indels
Additional features Phasing, 5mC methylation