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Takara Bio Shasta Single Cell instrument for automated single-cell capture and molecular analysis
Credit: Takara Bio USA
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Shasta™ Single Cell System

Shasta™ Single Cell System
Credit: Takara Bio USA

We have entered a new era in single-cell NGS analysis! Shasta technologies have surpassed the boundaries of existing methods, enabling the detection of events such as splicing isoforms, gene fusions, SNVs, and CNVs—in more cells than ever before.

Discover novel biomarkers through our complete, high-throughput solution: a state-of-the-art, automated dispensing and imaging platform combined with well-validated NGS library prep chemistries and bioinformatics tools for comprehensive data analysis.

•    Profile single-cell total RNA with full gene-body coverage using Shasta Total RNA-Seq technology
•    Amplify whole genomes for high-throughput single-cell DNA-seq with Shasta Whole-Genome Amplification technology
•    Analyze sequencing data and discover hidden biomarkers without being a bioinformatics expert with Cogent™ NGS tools

The Shasta™ Single Cell System includes an integrated optical system, allowing the identification of wells containing single cells for further processing.  The fluorescence filters enable further analysis by imaging cells stained with cellular dyes.

Equipped with Cogent bioinformatics tools for intuitive assay development, you can harness the Shasta system to develop pioneering assays of your own. Nanoliter-scale reactions contribute to cost savings on reagents and, when combined with Cogent software, you have the flexibility to develop novel NGS applications seamlessly.


Product Specifications
Cell throughput 1,500–100,000 cells
Applications mRNA-seq, total RNA-seq, WGA, and many other customized applications
Underlying reverse transcription method Oligo d(T) priming, random priming
Coverage Full gene-body
Biomarkers detected Genes, transcripts, splicing isoforms, gene fusions, lncRNAs, CNVs, SNVs
Analysis Freely available Cogent™ NGS tools
About Takara Bio USA
Takara Bio USA is a wholly-owned subsidiary of Takara Bio who manufactures and distributes research-use-only (RUO) kits, reagents and instruments for the life sciences, including next-generation sequencing (NGS), polymerase chain reaction (PCR), gene delivery, genome editing, single-cell and spatial transcriptomics, nucleic acid and protein purification, stem cell research and automated sample preparation.
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