ThruPLEX® DNA-Seq FLEX

ThruPLEX® FLEX provides a robust, user-friendly NGS library preparation system for processing challenging samples such as cfDNA and FFPE DNA. This technology yields DNA libraries with high molecular complexity and balanced GC representation while accommodating high inputs (≤200 ng) and input volumes (≤30 µl). All ThruPLEX products feature single-tube workflows that minimize hands-on time, the likelihood of process errors, and the risk of sample loss.

ThruPLEX FLEX technology is unmatched in its ability to provide even coverage across a wide range of GC content with high reproducibility and minimal bias, maximizing library diversity and data quality for genome-wide and targeted sequencing applications. With the patented ThruPLEX approach, leftover adapters are degraded post-ligation, avoiding the need for adapter dilution and intermediate cleanup steps required by other NGS library preparation methods. This approach allows for a straightforward workflow which can be completed in about two hours, with 15 min of hands-on time.

Overview
•    Construct Illumina libraries from 250 pg–200 ng inputs of fragmented dsDNA
•    Minimize sample loss with a fast, single-tube workflow
•    Maximize data quality with superior coverage uniformity and GC-rich region representation
•    Process challenging samples efficiently, including cfDNA and FFPE DNA, with robust template repair chemistry and flexible input volumes
•    Improve analysis of low-frequency variants through incorporation of unique molecular identifiers (UMIs) with ThruPLEX Tag-Seq FLEX
•    Multiplex up to 384 samples with compatible Unique Dual Index Kit sets
•    Process intact DNA inputs using enzymatic fragmentation with ThruPLEX DNA-Seq FLEX EF