TwistAmp® Liquid – The versatile PCR replacement
TwistAmp® Liquid provides recombinase polymerase amplification (RPA) assay reagents in a glycerol-stabilized, liquid kit format that will be familiar to anyone who carries out PCR and other enzyme-based reactions in the laboratory. The reagents are provided in separate tubes, and users can pipette out as much or as little of the enzyme mix as they want to make up RPA reaction volumes of their choosing, create a bulk master mix as the foundation for multiple reactions, or experiment with ratios of RPA components to optimize their assays.
TwistAmp® Liquid offers an alternative format to TwistDx’s flagship ambient temperature-stable lyophilized TwistAmp® kits, which are ideal for the development of field and site-based assays. The liquid format will make it easy for laboratories to use RPA routinely as a faster and more user-friendly replacement to PCR, sometimes even using the same PCR primers, and to develop customized assays without a thermocycler.
TwistAmp® Liquid range includes:
• TwistAmp® Liquid Basic
• TwistAmp® Liquid Basic RT
• TwistAmp® Liquid exo
• TwistAmp® Liquid exo RT.
| Features/Uses | TwistAmp® Liquid Basic | TwistAmp® Liquid | TwistAmp® Liquid | TwistAmp® Liquid |
Flexible reaction volume use | ✔ | ✔ | ✔ | ✔ |
Some flexible reagent component ratio use | ✔ | ✔ | ✔ | ✔ |
Single molecule detection | ✔ | ✔ | ✔ | ✔ |
Reagents for at least 100 reactions (dependent on volume used) | ✔ | ✔ | ✔ | ✔ |
Multiplexable | ✔ | ✔ | ✔ | ✔ |
Rapid DNA amplification | ✔ | ✔ | ✔ | ✔ |
Rapid RNA amplification | ✔ | ✔ | ||
Combined reverse transcription and rapid cDNA amplification in one step | ✔ | ✔ | ||
Real-time probe detection (proprietary exo probe design) | ✔ | ✔ | ||
Real-time fluorescence detection | ✔ | ✔ | ||
End-point gel electrophoresis detection | ✔ | ✔ | ||
down-stream applications (e.g. sub-cloning) | ✔ | ✔ |
Contact info@twistdx.co.uk for more information or visit the webpage www.twistdx.co.uk
